By Robert H. Rosa Jr., MD
This quantity is split into elements: half I, Ophthalmic Pathology; and half II, Intraocular Tumors: medical features. half I makes use of a hierarchy that strikes from basic to express to assist derive a differential prognosis for a particular tissue. half II is a compilation of chosen medical features of significance to the final ophthalmologist. Following half II are the yankee Joint Committee on melanoma 2010 staging varieties for ocular and adnexal tumors. This revised textual content includes various new pathologic and scientific pictures. significant revision 2011-2012.
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Additional info for 2011-2012 Basic and Clinical Science Course, Section 4: Ophthalmic Pathology and Intraocular Tumors (Basic & Clinical Science Course)
CHAPTER 3: Specimen Han dli ng . 31 Table 3-2 Common Stains Used in Ophthalmic Pathology Stain Material Stained: Color Example Hematoxylin and eos in (H& E) Nucleus: blu e Cytoplasm: red Glycogen and proteog lycans: magenta Acid mucopolysaccharide: bl ue Calcium: red Acid muco polysaccharide: blue Amylo id: orange, red-green dichroism Ac id-fast orga ni sms: red Fungal elements: black Gene ral tissue stain (Fig 3-2EI Descemet membrane (Fig 6-17EI Cave rn ous optic atrophy (Fig 15-1 0BI Band ke ratopathy Macular dystrophy (Fig 6-21CI Lattice dy strophy (Fig 6-23C, DI Atypica l m ycobacterium Fusarium (Fig 6-7B) Amyloid: pu rp le, violet Bacteria positive: blue Bacteria neg ative: red Lattice d ystrop hy Bacteria l infection Collagen: blue Granul ar dystrophy (F ig 6-22CI Red depos its Hemosi d erosis bulbi Lattice dystrophy Temporal artery elastic laye r Band ke rat opathy (Fig 6-1 2CI Periodic acid-Schiff (PAS) Alcian blue Alizarin red Colloidal iron Congo red Ziehl-Neelsen Gomori methenamine silver (GMSI Crystal violet Gram stain (tissue Brown & Brenn IB&BI or Brown & Hopps IB&Hl stain ) Masson trichrome Perls Prussian blue Thioflavin T (ThT) Verhoeff-van Gieson von Kassa Musc le: red Iron : blue Amyloid: flu orescent yellow Elastic fibers: black Calcium p hosp hate salts: bl ack CHAPTER 4 Special Procedures New technologies have contributed to improvements in the diagnosis of infectious agents and tumors as well as to the classification of tumors, especially the non-Hodgkin lymphomas (NHLs), childhood tumors, and sarcomas.
Which would indicate a poo r prognosis. Intraocular FNAB has also been ut ilized in the diagnosis of primary intraocular lymphoma, and the biopsy specimens can undergo flow cytometric analysis. immunocytologic analysis. cytokine analysis. or molecular biological analysis (using peR on both fixed and non fixed material). Special fixatives are used for cytology specimens. The procedure is performed under direct visualization through a dilated pupil. Iris tumors may be accessible for FNAB duri ng slit-lamp biomicroscopy.
18 • Ophtha lmic Pa t hology an d Intraocular Tum ors around the wound and actively contract, res ultin g in a volumetric decrease in the size of the wound. The eyelid and orbit are compartmentalized by intertwi ni ng fascial membranes enclosing muscular, tendinous, fa tty, lacrimal, and ocular tissues that a re disto rted b'Y'scarring. Exube rant cont racting d istorts m uscle ac tion, produci ng dysfunctional scars. The str~ated muscles of the orbic ularis oculi and ext raocular muscles a re made of te rmi nally d iffere nt iated cells th at do no t rege ne rate, but the via ble cells may hyp er troph y.
2011-2012 Basic and Clinical Science Course, Section 4: Ophthalmic Pathology and Intraocular Tumors (Basic & Clinical Science Course) by Robert H. Rosa Jr., MD